Coagulated Blood Genomic DNA Kit
2024-12-05Plant Genomic DNA Fast Kit
2024-12-05FFPE Tissue DNA Kit
Product Number: DNK3201
Shipping and Storage
- Buffer CB or Buffer IR may precipitate and precipitate at low temperatures. It can be dissolved again in a water bath at 37℃ for a few minutes to restore clarity and transparency. After cooling to room temperature, it can be used.
- Proteinase K is stored in a ready to use glycerol buffer and transported at room temperature. After receipt, store at room temperature not exceeding 25℃ for at least 6 months, at 4℃ for 12 months, and at -20℃ for 2 years.
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | DNK3201 50 times | DNK3202 100 times | DNK3203 200 times |
| Balance Buffer | RT | 5 ml | 10 ml | 20 ml |
| Buffer FTL | RT | 11 ml | 20 ml | 40 ml |
| Buffer CB | RT | 11 ml | 20 ml | 40 ml |
| Buffer IR | RT | 25 ml | 50 ml | 100 ml |
| Buffer WB | RT | 13 ml | 25 ml | 50 ml |
| Buffer EB | RT | 15 ml | 15 ml | 15ml×2 |
| Proteinase K | -20℃ | 1.5ml | 3ml | 6ml |
| Adsorption column AC | RT | 50 | 100 | 200 |
| Collection tube (2ml) | RT | 50 | 100 | 200 |
Description
Formalin fixed or paraffin embedded tissues are rapidly lysed by unique Buffer FTL heat treatment and Proteinase K to release genomic DNA. The genomic DNA is then selectively adsorbed onto the silica matrix membrane in a high dissociation salt state in a centrifuge column, followed by a series of rapid rinsing centrifugation steps. Buffer IR and Buffer WB remove impurities such as cell metabolites and proteins, Finally, low salt Buffer EB elutes pure genomic DNA from the silica matrix membrane.
Features
- The silicon matrix membrane inside the centrifugal adsorption column is entirely made of specially designed adsorption membranes from world-renowned imported companies, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
- No toxic reagents such as phenol are required, and no steps such as ethanol precipitation are required.
- Fast and simple, the operation of a single sample can generally be completed within 30 minutes.
- Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.7~1.9 and a length of up to 30kb-50kb. It can be directly used for PCR, Southern blot, and various enzyme digestion reactions.
Application
Suitable for rapid extraction of DNA from various formalin fixed and paraffin embedded tissues
