Blood Mitochondrial DNA Extraction Kit
2025-07-09Product Number: DNK51
Shipping and Storage
2-8℃, among which DNase I and RNase A need to be stored at -20℃ with a shelf life of one year.
Components
| Component | 50T | 100T |
| DNase I | 12mg | 2×12mg |
| RNase A | 500μL | 2×500μL |
| 10×Blood lysis buffer | 100mL | 2×100mL |
| Cell lysis buffer | 50mL | 100mL |
| Mt Wash buffer | 25mL | 50mL |
| DNAase reaction buffer | 6mL | 12mL |
| Mt lysis buffer | 10mL | 20mL |
| Buffer PP | 7.5mL | 15mL |
| Poly Carrier | 0.5mL | 1mL |
| Buffer TE | 25mL | 50mL |
Description
This kit can be used to isolate complete and purified mitochondrial DNA from blood. The key to extracting mitochondrial DNA is to remove nuclear DNA as much as possible. This reagent kit uses differential centrifugation to obtain relatively pure mitochondria, and then uses multiple steps such as DNA enzyme digestion and lysis buffer system to remove nuclear DNA, finally obtaining pure mitochondrial DNA. Can be used for experiments such as PCR that require high purity.
