Manual

Product Number: RNK0501

Shipping and Storage

1. After adding anhydrous ethanol to Buffer WS1 and Buffer WS2/3, they can be stored at room temperature for one month. If they need to be stored for a longer period of time, please store them at 4℃. However, before use, they should be restored to room temperature first
2. Buffer WS2/3 may precipitate crystals after using ethanol for a few days, which does not affect its use. Simply do not absorb the crystals and use the supernatant.
3. Transportation is carried out at room temperature without affecting the effectiveness of use
4. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Component

Description

In recent years, extensive research on RNA interference and regulatory small RNAs urgently requires a reagent kit that can effectively extract RNA of around 15-30 nucleotides in size (including siRNA and miRNA). However, traditional RNA extraction methods such as silica gel membranes cannot effectively adsorb and recover small molecule RNA, while phenol/guanidine extraction and ethanol precipitation cannot effectively precipitate and recover small molecule RNA. This reagent kit uses a unique Buffer LY/BD, B-mercaptoethanol to rapidly lyse cells and inactivate cell RNA enzymes, with strong organic extraction to remove proteins and DNA. RNA, including small molecules, is adsorbed onto a special silicon-based membrane in a centrifuge column and then subjected to a series of rapid rinsing centrifugation steps. The rinsing solution further removes impurities such as cell metabolites and proteins, and finally, pure RNA is eluted from the silicon-based membrane using a low salt elution solution.

Features

1. The silicon matrix membrane inside the centrifugal adsorption column is entirely made of specially designed adsorption membranes from world-renowned imported companies, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
2. It also does not require steps such as ethanol precipitation that are prone to losing small RNA molecules.
3. Fast and simple, the operation of a single sample can generally be completed within 30 minutes.
4. Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.9~2.0 and almost no DNA residue. It can be used for RNAi, RT-PCR, Northern blot, and various experiments.

Application

Suitable for rapid extraction of miRNAs and other small RNAs from various cell tissues

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