Manual

Product Number: RNK0301

Shipping and Storage

1. Therefore, transportation and storage are carried out at room temperature (15℃-25℃). The TRIpure can be transported at room temperature, and can be stored for a long time in a dark place at 4℃ after receipt. Storage at room temperature for 3 months does not affect the quality of use.
2. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly after use.

Component

This reagent kit (excluding low-temperature components) does not affect its effectiveness when stored at room temperature for 12 months.

Description

Improved guanidine isothiocyanate/phenol one-step method (TRIzol method) is used to lyse cells and inactivate RNAses. Total RNA is then selectively adsorbed onto the silica matrix membrane in a highly ionized salt state, followed by a series of rapid rinsing centrifugation steps to remove cell metabolites, proteins, and other impurities from the protein solution and rinse solution. Finally, low salt RNase free water is used to elute pure RNA from the silica matrix membrane.

Features

1. The silicon matrix membranes inside the centrifugal adsorption column are all made of specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability.
2. Combining the advantages of good stability, high purity, and convenient and fast centrifugation column of guanidine isothiocyanate/phenol one-step reagent, it does not require isopropanol precipitation and ethanol washing process. RNA can be directly eluted from the centrifugation column to avoid the problem of excessive drying and difficult dissolution.
3. TRIpure Reagent corresponds to ThermoFisher/Invitrogen's TRIzol, which can effectively eliminate genomic contamination. The effect is consistent with the import.
4. Multiple rinsing and deproteinization processes result in higher purity of RNA extraction.
5. Effectively removed the content of 5S in total RNA and improved purity.

Note

1. This reagent kit exhibits excellent inhibition of RNA enzymes, and all centrifugation steps can be performed at room temperature unless otherwise specified.
2. The TRIpure Reagent and Buffer PE contain irritating and harmful compounds. When operating, latex gloves should be worn to avoid contact with skin, eyes, and clothing. If it comes into contact with the skin or eyes, rinse with plenty of water or physiological saline.
3. Considering environmental protection issues, this reagent kit does not contain commonly used laboratory reagent chloroform, and you need to prepare your own chloroform before use. But if chloroform is really difficult to obtain, it can also be avoided.
4. Conventional agarose gel electrophoresis and denaturing gel electrophoresis can be used to analyze the quality of RNA. A good RNA product should show two distinct dominant ribosomal RNA bands after electrophoresis, namely~5Kb (28S) and~2Kb (18S), with a band brightness ratio of approximately 2:1.Sometimes~0.1kb and 0.3Kb (5S, tRNA) bands can also be seen. But sometimes it is normal to see 4-5 bands according to different species, such as certain plant tissues. If the precursor of RNA is immature or uneven nuclear RNA or small nuclear RNA is extracted, discontinuous high molecular weight bands between 7Kb and 15Kb may also be seen.
5. After adding TRIpure Reagent homogenate and before adding chloroform, the sample can be stored at -60℃ -70℃ for more than one month.
6. If extracting bacterial RNA, it is recommended to use the EASYspin Plus Bacterial Fast RNA Kit (RNK4302).

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