Smartarose 4B
2025-10-24Product Number: S080203
Shipping and Storage
- Packaging: PP material reagent bottle sealed packaging, with a specification of 25mL. Please consult for other packaging.
- Storage: 2-8℃, 20% ethanol.
- Shelf life: 24 months.
Component
| Component | S080203 |
| Ni-NTA Agrose 6 Fast Flow | 25mL |
Description
Ni NTA SweAgorase 6 Fast Flow is a metal ion chelating affinity chromatography separation medium formed by binding NTA ligands chelated with nickel ions onto agarose microspheres. Fixed metal ion affinity chromatography (NTA) utilizes the interaction between Ni2+and the side chains of certain amino acids (mainly histidine, with small amounts of cysteine and tryptophan) on proteins for separation and purification.
Features: Wide range of use, simple operation, suitable for gravity columns and pre installed columns;
High flow rate, high loading capacity, low Ni2+detachment, and wide compatibility of reagents;
Multiple selection can chelate various metal ions for use, usually Zn2+, Ni2+, Cu2+or Co2+.
Performance
| Indicator | Description |
| Product Content | 66% (v/v) agarose gel, stored in 20% ethanol |
| Gel type | 6% cross-linked agarose gel |
| Ligands | -NTA-Ni2+ |
| Particle size range (μm) | 45 - 165 |
| Average particle size (μm) | 90 |
| Combined with load capacity | 20-50 mg (His tagged protein)/mL medium |
| Ligand density (μmol/mL) | ~ 25 |
| Operating flow rate (cm/h) | ≤600 |
| Pressure resistance (MPa) | 0.3 |
| Chemical stability range | Commonly used aqueous buffer solutions; 0.01 M hydrochloric acid, 0.1 M sodium hydroxide (1 week); 1 M sodium hydroxide, 70% acetic acid (1 day); 2% SDS (1 hour); 30% 2-propanol (30 minutes) |
