Goldview Nucleic Acid Gel Stain
2026-07-03
SafeRed Nucleic Acid Gel Stain
2026-07-03SafeGreen Nucleic Acid Gel Stain
Product Number: ST04
Shipping and Storage
Store away from light at 4°C or room temperature for 24 months.
Description
SafeGreen Nucleic Acid Gel Stain is a kind of new generation of fluorescent nucleic acid gel stain designed to replace the highly toxic ethidium bromide (EtBr). The Ames test confirmed that SafeGreen are nonmutagenic at concentrations well above their working concentrations used for gel staining. SafeGreen Nucleic Acid Gel Stain are highly sensitive than EtBr either as precast gel stains or post gel stains.
SafeGreen has UV absorption between 250nm and 300nm and a strong absorption peak centered around 500nm and is compatible with either a 254nmUV transilluminator or a gel reader equipped with visible light excitation(such as a 488nm laserbased gel scanner or a Dark Reader).
SafeGreen Nucleic Acid Gel Stain 10,000X in DMSO is a concentrated SafeGreen solution that can be diluted 10,000 times for use in precast gel staining for ~3,300 times for use in post gel staining according to the procedures described below. One vial(0.5ml) of 10,000X solution can be used to prepare at 100 precast minigels or post-stain at least 100 minigels.
Gel staining with SafeGreen is compatible with downstream applications such as gel extraction and cloning. SafeGreen is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.
Features
- Safe and non-toxic: The unique oily macromolecules prevent it from penetrating the cell membrane and entering the cell. This dye does not have EB carcinogenic mutagenicity.
- Good migration rate: EB small molecules quickly escape from the gel, so EB can easily cause small DNA fragments to be unclear. Our large molecule Safe Green Stain completely overcomes this.
- Accurate quantification: Suitable for determining and quantifying the size of nucleic acid molecules, EB is not accurate in quantifying small DNA fragments.
- Uniform dyeing: the brightness of the negative and positive ends of the whole gel is the same. EB can cause the overall background of the glue to be slightly higher, often appearing as a yin and yang background (the background of the glue is partly bright and partly dark); Long term and long-distance electrophoresis of EB will result in a corresponding decrease in EB signal intensity. Our macromolecular Safe Green Stain completely overcomes this.
- High sensitivity: suitable for electrophoretic staining of various sizes of fragments, with less impact on nucleic acid migration than SYBR Green I.
- High stability: heat resistant, can be added in buffer solution, and gel can be dissolved at 100 ℃ to prevent dye from not being fully mixed. It is suitable for preparing agarose gel by microwave or other heating methods.
- Strong light resistance: It can be stored at room temperature for 24 months under daily light exposure in the laboratory.
- Good signal-to-noise ratio: The sample has a strong fluorescence signal, a low background signal, and a fluorescence brightness more than ten times that of EB. The brightness can be observed with the naked eye to be significantly stronger than EB.
- Easy to operate: The usage is exactly the same as EB, and the dye does not degrade during the pre gel and electrophoresis process; The staining process after electrophoresis only takes 30 minutes and does not require decolorization or rinsing.
- Wide scope of application: suitable for agarose gel or polyacrylamide gel electrophoresis; Can be used for staining dsDNA, ssDNA, or RNA.
- Perfect compatibility: Safe Green Stain is compatible with all ultraviolet g el transmission instruments; Compatible with all blue and visible light instruments. It has similar spectral characteristics to EB, and does not need to change the filter and observation device: standard EB filter or SYBR filter are applicable, and the best excitation can be obtained near 300 nm ultraviolet light by using the same ultraviolet gel transmission instrument as EB.
