Manual

Product Number: DTL1148

Shipping and Storage

1. Store below 30°C. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used the all components should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. It is not recommended to repeat the freeze-thaw cycle more than 7 times.
4. Date of manufacture and term of validity: see the label.

Component

Component	48T
BVDV RT-PCR Master Mix	Lyophilized powder ×1 Bottle
Exogenous Internal Control	Lyophilized powder ×1 Bottle
Positive Control	100μL
Negative Control	1mL
Redissolved Diluent	1.5mL

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, reverse transcriptase, primers, probes, dNTPs, and Mg²⁺.

Description

Bovine viral diarrhea is an infectious disease caused by bovine viral diarrhea virus (BVDV), which can cause many clinical symptoms and some diseases, including reproductive disorders, respiratory syndrome, congenital defects, enteritis, persistent infection (PI) and mucosal disease (MD). In addition to cattle, susceptible animals also include camels, sheep, goats, pigs, deer and a variety of wild ruminants. The secretions, excrement, blood and spleen of sick cattle contain viruses, which are transmitted by direct or indirect contact.

This kit is suitable for detecting bovine viral diarrhea virus nucleic acid in bovine tissue samples, nasal swabs and other specimens, and is suitable for auxiliary diagnosis of bovine viral diarrhea virus infection.

Application

This kit uses TaqMan probe real-time fluorescence PCR technology to design a pair of bovine viral diarrhea virus-specific primers, combined with a specific probe, and uses fluorescence PCR technology to perform in vitro amplification and detection of bovine viral diarrhea virus nucleic acid, which is used for clinical etiological diagnosis of suspected infected samples.

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