Manual

Product Number: DTL0408

Shipping and Storage

1. Store below 30℃. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used the all components should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. It is not recommended to repeat the freeze-thaw cycle more than 7 times.
4. Date of manufacture and term of validity: see the label.

Component

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, reverse transcriptase, primers, probes, dNTPs, and Mg²⁺.

Description

This kit uses real-time fluorescence PCR detection technology to achieve qualitative detection of Hepatitis E virus in serum or plasma samples by using a pair of specific primers and a specific fluorescent probe of Hepatitis E virus. The kit is provided with an internal control, which can monitor whether there is PCR inhibitor in the sample to be tested by detecting whether the Internal control is normal or not, so as to avoid false negative PCR.

Application

This kit is suitable for the qualitative detection of Hepatitis E virus nucleic acid (HEV RNA) in human serum samples in vitro, and the test results are used for the auxiliary diagnosis of Hepatitis E.The test results are not the only indicators for the evaluation of the patient's condition, and the condition must be comprehensively analyzed in combination with the patient's clinical manifestations and other laboratory tests.

Applicable instruments

Real-time fluorescence PCR instrument with FAM, VIC channels.

Specimen collection

1. Applicable sample type: Serum specimens.
2. Sample collection: Use a sterile syringe to draw 2mL of the subject's venous blood and inject it into a sterile centrifuge tube. Leave it at room temperature for no more than 4 hours. Centrifuge at 2000rpm for 5 minutes. Take the upper serum (be careful not to bring in red blood cells) and transfer it to another sterile centrifuge tube for use.
3. Sample storage and transportation: The collection or processing sample should not exceed 24 hours under the conditions of 2℃ ~ 8℃. If long-term preservation is needed, it should be stored below -70°C, and the freezing fusion should not exceed 3 times.

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