Manual

Product Number: DTL0716

Shipping and Storage

1. Store below 30°C. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used the all components should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. It is not recommended to repeat the freeze-thaw cycle more than 7 times.
4. Date of manufacture and term of validity: see the label.

Component

Component	48T
IAV/IBV/RSV RT-PCR Master Mix	Lyophilized powder ×1 Bottle
Positive Control	100μL
Negative Control	1mL
Redissolved Diluent	1.5mL

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, reverse transcriptase, primers, probes, dNTPs, and Mg²⁺.

Description

This kit uses the multiplex fluorescent PCR detection technology to to design specific primers and Taqman fluorescent probes for the conserved regions of influenza A virus, influenza B virus, respiratory syncytial virus, so as to realize the qualitative detection of influenza A virus, influenza B virus, respiratory syncytial virus RNA in oropharyngeal swabs. The kit is provided with an Internal Control（IC）, which can monitor whether there is PCR inhibitor in the sample to be tested by detecting whether the internal control is normal or not, so as to avoid false negative PCR.

Application

Influenza viruses are divided into four types: A, B, C, and D. Influenza viruses can cause infection and disease in a variety of animals such as humans, poultry, pigs, horses, and bats, and are the causative agent of human and animal diseases such as human influenza, avian influenza, swine influenza, and equine influenza. Typical clinical symptoms of these diseases are acute high fever, generalized pain, significant fatigue and respiratory symptoms. Influenza viruses are mainly spread through droplets in the air, contact between susceptible and infected people, or contact with contaminated items. Generally, autumn and winter are the periods of high incidence.

Respiratory syncytial virus (RSV) belongs to the genus Pneumovirus of the family Paramyxoviridae and has only one serotype. It mainly causes lower respiratory tract infections such as bronchiolitis and pneumonia in infants under 6 months of age, and upper respiratory tract infections such as rhinitis and colds in older children and adults.

This kit is suitable for detecting Influenza A virus,Influenza B virus and Respiratory Syncytial Virus in samples such as oropharyngeal or nasal swab sample, and is used for auxiliary diagnosis of Influenza A virus,Influenza B virus and Respiratory Syncytial Virus infection.

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