Manual

Product Number: DTL1412

Shipping and Storage

1. Store below 30°C. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used the all components should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. It is not recommended to repeat the freeze-thaw cycle more than 7 times.
4. Date of manufacture and term of validity: see the label.

Component

Component	48T
NPTII PCR Master Mix	Lyophilized powder ×1 Bottle
Positive Control	100μL
Negative Control	1mL
Redissolved Diluent	1.5mL

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, reverse transcriptase, primers, probes, dNTPs, and Mg²⁺.

Description

This kit utilizes the TaqMan probe-based real-time fluorescent PCR technique. A pair of specific primers and a corresponding specific probe are designed targeting the NPTII gene sequence to amplify and detect the DNA of the NPTII gene in vitro by fluorescent PCR, so as to achieve rapid detection.

Application

Neomycin Phosphotransferase II (NPT II) confers resistance to aminoglycoside antibiotics (e.g., kanamycin, neomycin) in host organisms. As a dominant selectable marker for genetically modified plants, it is widely used in plant genetic engineering. This kit is suitable for the detection of the NPTII gene in transgenic plants and is used to screen for the presence of NPTII in plant samples.

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