Manual

Product Number: DTL1224

Shipping and Storage

1. Store below 30°C. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used the all components should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. It is not recommended to repeat the freeze-thaw cycle more than 7 times.
4. Date of manufacture and term of validity: see the label.

Component

Component	48T
VHSV RT-PCR Mater Mix	Lyophilized powder ×1 Bottle
Exogenous Internal Control	Lyophilized powder ×1 Tube
Positive Control	100μL
Negative Control	1mL
Redissolved Diluent	1.5mL

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, reverse transcriptase, primers, probes, dNTPs, and Mg²⁺.

Description

This kit designs specific primers and probes for viral hemorrhagic septicemia virus genes, and uses one-step fluorescent RT-PCR technology to perform in vitro amplification and detection of viral hemorrhagic septicemia virus RNA for etiological diagnosis of suspected infected samples.

Application

Viral hemorrhagic septicemia is a highly fatal disease caused by viral hemorrhagic septicemia virus (VHSV), which belongs to the genus Rhabdovirus of fishes. VHSV contains 3 serotypes and 4 genotypes, and there is no correlation between serotypes and genotypes. The disease first became prevalent in continental Europe, mainly infecting rainbow trout, flatfish, and several wild marine fish. The disease shows obvious clinical symptoms during the acute infection period: sick fish are lethargic, the skin turns black, the eyes are protruding, anemia, bleeding at the base of fin rays, gills, eyes, and skin, abnormal swimming, and abdominal edema. The main transmission route of the virus is contact with other infected fish or contaminated water, pollutants or through fish-eating birds.

This kit is suitable for detecting viral hemorrhagic septicemia virus RNA in specimens such as the brain, liver, kidney, spleen, gills and other tissues of fish, and ovarian fluid of female fish, and is suitable for auxiliary diagnosis of viral hemorrhagic septicemia virus infection.

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