Manual

Detection Kit (Dual Fluorescent PCR Method)

Product Number:DTK515

Shipping and Storage

1. -20℃± 5℃, stored in the dark, transported, and subjected to repeated freeze-thaw cycles no more than 5 times, with a validity period of 12 months.
2. The above specimens can be stored at -20 ℃ in the short term and -70 ℃ in the long term, but cannot exceed 6 months. The specimens should be transported in 2-8 ℃ ice packs, and repeated freezing and thawing are strictly prohibited.

Component

Note: Different batches of reagents cannot be mixed.

Description

Hand, foot, and mouth disease is a common childhood illness caused by enteroviruses, mostly resulting in fever and water rash on the mouth, palms, and soles of the feet. A small number of patients may experience symptoms such as meningitis, encephalitis, neurogenic pulmonary edema, myocarditis, and even death. The enteroviruses that cause hand, foot, and mouth disease include Coxsackievirus, novel enteroviruses, and enteroviruses. Currently, laboratory diagnostic methods mainly include virus isolation and nucleic acid testing. Hand foot mouth disease is an acute infectious disease caused by enteroviruses (Coxsackie A group 16, enterovirus 71 is the most common). It mostly occurs in preschool children, especially in the age group under 3 years old, with the highest incidence rate.

This kit is suitable for detecting enterovirus EV71 and Coxsackievirus CA16 RNA in stool or diarrhea samples, and is suitable for auxiliary diagnosis of enterovirus EV71 and Coxsackievirus CA16 infections. The test results are for reference only.

Application

This kit uses TaqMan probe method for real-time fluorescence PCR technology, designs a pair of enterovirus EV71 and Coxsackievirus CA16 specific primers, and combines them with a specific probe to amplify and detect the RNA of enterovirus EV71 and Coxsackievirus CA16 in vitro using fluorescence PCR technology, which is used for pathogen diagnosis of suspected infected individuals in clinical practice.

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