Klebsiella pneumoniae nucleic acid detection kit (fluorescence PCR method)
2025-07-11
EXO Lyophilized Essence Serum
2025-07-11Streptococcus pneumoniae serotyping nucleic acid multiplex real-time fluorescence PCR detection kit
Product Number: DTK811
Shipping and Storage
- Transportation: The reagent kit must be transported under frozen conditions.
- Storage: Store at -20 ± 5℃ and avoid repeated freezing and thawing. The freeze-thaw cycle of the reagent kit shall not exceed 7 times.
- Validity period: 12 months, please use within the validity period.
Component
| Component | 25T |
| Nucleic acid amplification reaction solution Mix-A | 450μL |
| Nucleic acid amplification reaction solution Mix-B | 450μL |
| Nucleic acid amplification reaction solution Mix-C | 450μL |
| Nucleic acid amplification reaction solution Mix-D | 450μL |
| Nucleic acid amplification reaction solution Mix-E | 450μL |
| Nucleic acid amplification reaction solution Mix-F | 450μL |
| Enzyme mixture | 300μL |
| Positive control | 200μL |
| Negative control | 1000μL |
Note: The ingredients of different batches of reagent kits cannot be mixed!
Description
This kit uses real-time fluorescence PCR technology and is suitable for serum typing of Streptococcus pneumoniae extracted from pure cultures or single colonies obtained from clinical and food samples, as well as pure cultures of preserved strains. Each reaction system contains specific primers and fluorescent probes for detecting the target gene. By collecting the fluorescent signal generated by PCR amplification, qualitative detection of Streptococcus pneumoniae serum typing nucleic acid can be quickly completed.
Application
This kit is designed for the qualitative serotyping of Streptococcus pneumoniae from pure cultures or single colonies isolated from clinical specimens, food samples, or preserved bacterial strains. It covers 24 common serotypes, including 1, 2, 3, 4, 5, 6B/6D, 6A/6B, 7F/7A, 8, 9V/9A, 9L/9N, 10A, 11A/11D/11E, 12F/44, 14, 15B/15C, 17F, 18C/18F/18B/18A, 19A, 19F, 20, 22F, 23F, and 33A/33F/37. The experimental results are intended for basic research purposes only and should not be used as a basis for clinical diagnosis.
Streptococcus pneumoniae often resides in the nasopharyngeal cavity of normal individuals and is mostly non pathogenic, with only some having pathogenicity. It can cause diseases such as lobar pneumonia, peritonitis, and pleurisy.
Specimen collection
Pure cultures or single colonies obtained from clinical and food samples, as well as DNA samples extracted from pure cultures that preserve bacterial strains, can all be tested using this kit.
