Manual

Product Number: S011123

Shipping and Storage

The entire set of reagents can be transported at room temperature; Protein standard (BSA) is stored at 2-8℃ and has a shelf life of 12 months. After preparing the protein standard solution, it needs to be stored at -20℃ and used within 6 months. The remaining reagents are stored at room temperature and have a shelf life of 12 months.

Component

Description

There are currently two most commonly used methods for protein concentration quantification, namely Bradford method and BCA method. The basic principle of BCA method for quantitative detection of proteins is based on the biuret reaction, which means that under alkaline conditions, Cu²⁺is reduced by proteins to Cu⁺, and then Cu⁺undergoes chelation reaction with BCA (Bicinchonic acid, a color developer). Every two molecules of BCA chelate one Cu⁺, generating a purple water-soluble complex. This substance has the highest absorption value at 562nm, and the color depth is proportional to the protein concentration within a certain range. Therefore, it can be used for quantitative detection of proteins. The protein concentration has a good linear relationship in the concentration range of 50-2000μg/mL.

This method is not affected by the majority of chemical substances in the sample and is compatible with high concentrations of detergents in the sample, including SDS with concentrations up to 5%, Triton X-100 with 5%, Tween-20, 60, 80 with 5%, etc. However, chelating agents and high concentrations of reducing agents can affect the detection results. It is necessary to ensure that there is no EGTA in the sample, EDTA concentration is less than 10mM, DTT is less than 1mM, and β- mercaptoethanol is less than 1mM. If the sample contains chelating agents or reducing agents, other products of our company, S011125 Bradford protein quantification detection kit, can be considered.

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