SYBR Green I Nucleic Acid Gel Stain (20x, for qPCR)
2026-07-03
Goldview Nucleic Acid Gel Stain
2026-07-03SYBR Green I Nucleic Acid Gel Stain (10000x)
Description
Sybr Green I and Sybr Green II nucleic acid colorants are new generation fluorescent nucleic acid gel colorants designed to replace the highly toxic ethidium bromide (EtBr). Sybr Green I is non-toxic and more sensitive than EtBr. Gel can be visualized under ultraviolet or visible light.
SYBR Green I nucleic acid gel stain was developed specifically for reduced mutagenicity—to be safer than ethidium bromide for staining DNA in agarose or acrylamide gels. SYBR gel stain is not only less mutagenic than ethidium bromide, but SYBR gel stain’s detection sensitivity is better than that of ethidium bromide. SYBR gel stain comes as a premixed solution that can be used just like an ethidium bromide solution, either in the gel during the electrophoresis run or as a post-run stain. Bound to nucleic acids, the green-fluorescent SYBR gel stain has fluorescence excitation maxima at ~280 and ~502 nm, and an emission maximum at ~530 nm.
Component
The dye is supplied as a 10,000x solution in dimethyl sulfoxide (DMSO). One ml of solution prepares 10 liters of Staining Solution, sufficient for 100 mini gels.
Features
- Safety: Sybr Green is nontoxic and noncarcinogenic.
- Ultra-sensitivity: It allows the visualization of as little as 20pg dsDNA, around 5-10 times more sensitive than EtBr under UV and 8-20 times more sensitive than EtBr in Visible Light.
- Convenience: NO need to rinse or wash gels. Add stain before load samples. Visualize gels under UV or Visible Light to avoid UV damage on DNA/RNA.
- Wide range: suitable for agrose gel or PAGE.
- No impact for the next experiments such as RT, PCR, enzyme digestion, and ligation.
- Strong signal and no background
- Low cost: 1 ml Sybr Green I Nucleic Acid Stain is sufficient to load 10,000 samples.
Note
- Do not run gels over 2h. Or smeared bands appeared because Sybr Green I Stain will dissociate from DNA/RNA.
- Sybr Green I Stain can dissociate from nucleic acids in ethanol.
- Please stain nucleic acid in the gel or after electrophoresis to check the exact molecular weight of fragments when compared with molecular weight markers.
- Please stain DNA with DNA Stain and RNA with RNA Stain.
- Please use EP tubes and other plastic wares in Sybr Green I Stain storage, dilution, and staining. Sybr Green I Stain can bind to glassware.
