SYBR Green I nucleic acid gel stain was developed specifically for reduced mutagenicity—to be safer than ethidium bromide for staining DNA in agarose or acrylamide gels. SYBR gel stain is not only less mutagenic than ethidium bromide, but SYBR gel stain’s detection sensitivity is better than that of ethidium bromide. SYBR gel stain comes as a premixed solution that can be used just like an ethidium bromide solution, either in the gel during the electrophoresis run or as a post-run stain. Bound to nucleic acids, the green-fluorescent SYBR gel stain has fluorescence excitation maxima at ~280 and ~502 nm, and an emission maximum at ~530 nm.
The dye is supplied as a 10,000x solution in dimethyl sulfoxide (DMSO). One ml of solution prepares 10 liters of Staining Solution, sufficient for 100 mini gels.