Yeast Transformation Kit (Saccharomyces cerevisiae)
2025-12-12
Epstein-Barr Virus (EBV) Nucleic Acid Detection Kit (Real-Time PCR Method)
2025-12-16DNA Methylation Kit
Product Number: EK0227
Shipping and Storage
The adsorption column DF is stored at 2-8℃, while the remaining components are stored at room temperature (15-30℃).
Component
| Component | 50T |
| Conversion solution CR | 5×1mL |
| Buffer solution CL | 30mL |
| Buffer solution MD | 0.4mL |
| Buffer DB | 10mL |
| Rinse solution WB (concentrated solution) | 10mL |
| Rinse buffer 1 (concentrated solution) | 13mL |
| Rinse buffer 2 (concentrated solution) | 15mL |
| Eluent solution | 4mL |
| Column equilibrium liquid PS | 10mL |
| Adsorption Column DF | 50Pcs |
| Collection tube | 50Pcs |
Description
The basic principle of this kit is that after treatment with bisμLfite, DNA can be converted from unmethylated cytosine to uracil, while methylated cytosine remains unchanged.
This reagent kit adopts an alkaline denaturation treatment method, which can denature DNA double stranded under mild conditions, ensuring DNA integrity while improving conversion efficiency. The conversion efficiency can reach over 99.5%. At the same time, by combining the conversion product with the adsorption column, purified DNA is recovered from the bisμLfite modified solution. The recovered DNA has high purity and good integrity, and can be directly used for downstream experiments such as sequencing, methylation PCR detection, and chip analysis.
Features
- High conversion efficiency: The conversion efficiency of unmethylated cytosine is greater than 99.5%
- High nucleic acid integrity: minimal damage to nucleic acid, beneficial for downstream testing
- High recovery rate: recovery rate greater than 80%
- High sample compatibility: compatible with DNA and cfDNA with an input range of 100pg-2ug.
Materials required but not supplied
Anhydrous ethanol, PCR tube, EP tube, centrifuge.
