Manual

Product Number: DTL0213

Shipping and Storage

1. Store below 30°C. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used the all components should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. It is not recommended to repeat the freeze-thaw cycle more than 7 times.
4. Date of manufacture and term of validity: see the label.

Component

Component	48T
JEV RT-PCR Master Mix	Lyophilized powder ×1 Bottle
JEV Positive Control	100μL
JEV Negative Control	1mL
Redissolved Diluent	1.5mL

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, reverse transcriptase, primers, probes, dNTPs, and Mg²⁺.

Description

The kit uses one-step reverse transcription polymerase chain reaction (RT-PCR) combined with Taqman technology to design specific primers and specific fluorescent probes for herpes simplex virus and the conserved region of human endogenous gene RNaseP sequence, so as to realize the qualitative detection of Japanese encephalitis virus in patient serum samples.

Application

Japanese encephalitis virus (JEV) is a single-stranded positive-sense RNA virus that can cause zoonotic infectious diseases through mosquitoes.The disease is an acute infectious disease mainly caused by lesions of the central nervous system. It has an acute clinical onset and has symptoms such as high fever, disturbance of consciousness, convulsions, tonic spasms and meningeal irritation. Severe cases often have sequelae after recovery, which is extremely harmful to children.

This kit is suitable for detecting Japanese encephalitis virus RNA in serum samples, and is suitable for auxiliary diagnosis and epidemiological investigation of Japanese encephalitis virus infection. The test results are for reference only.

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