Supplied by bulk package

Description of the 2x PCR master mix

This PCR master mix contains the Taq DNA Polymerase, dNTPs, and optimized buffer but without any dye in it. This Taq Mastermix is provided at 2× concentration and used at 1× concentration by adding the template, primer, and H2O. It has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity. The extending speed is 1-2 kb/min. There is an "A" on the 3′end. The PCR product can be cloned in a TA vector.

Quality Control

This product has passed the following quality control assays: functional absence of double- and single-stranded endonuclease activity; >90% homogeneous by SDS gel electrophoresis. Each lot of our Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA 

Application 

• PCR
• Primer Extension
• Microarray Analysis
• High-Throughput PCR

Storage: Store at -20°C

Shipping with blue ice

Reaction Mixture Set Up

Template <0.5 μg

Forward Primer (10 μM) 1 μl

Reverse Primer (10 μM) 1 μl

2×TransTaq PCR SuperMix 25 μl

ddH2O to final volume 50 μl

No repeated freezing and thawing and split charging for good amplification.

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