This Taq DNA polymerase we supplied by Bulk package

Taq DNA polymerase is isolated from the E. coli cloning Thermus aquaticus. The molecular weight of the product is approximately 94 kDa. EasyTaq DNA polymerase has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity. The extending speed is 1-2 kb/min. There is an "A" on the 3′end. The PCR product can be cloned in TA vector.

Conc. 5 U/μl

Store at -20°C

Characteristics

1. high-sensitivity

2. high amplification efficiency

Unit Definition

One unit of Platinum Taq DNA Polymerase High Fidelity incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.

Quality Control

This product has passed the following quality control assays: functional absence of double- and single-stranded endonuclease activity; >90% homogeneous by SDS gel electrophoresis. Each lot of EasyTaq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA.

Storage Buffer

20 mM Tris-HCl (pH 8.0),0.1 mM EDTA,1 mM DTT,100 mM KCl,Stabilizers,50% glycerol.

10X PCR reaction Buffer

500 mM KCl,100 mM Tris-Cl (pH8.5 at 25℃),1% Triton X-100,15 mM MgCl2

Reaction Mixture Set Up

Component Volume Final Concentration

Template DNA <0.5 ug as required

Forward Primer (10 μM) 1 μl 0.2-0.4 μM each

Reverse Primer (10 μM) 1 μl 0.2-0.4 μM each

10×PCR reaction Buffer 5 μl 1×

2.5 mM dNTPs 4 μl 0.2 mM

Taq DNA polymerase 0.5μl 2.5 unit

ddH2O to final volume 50μl Not applicable

Recommended thermal cycling conditions

94℃ 2-5 min

94℃ 30 sec

50-60℃ 30 sec 30-35 cycles

72℃ 1-2 kb/min

72℃ 5-10 min

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