Manual

Product Number: RNK7201

Shipping and Storage

1. This reagent kit is transported and stored at room temperature.
2. To avoid the volatilization, oxidation, and pH changes of reagents exposed to air for a long time, the lid of each solution should be promptly closed after use.

Components

Component	RNK7201 50 Preps
Buffer RC	20mL
Buffer RW	10mL
	Add anhydrous ethanol as indicated on the bottle label before the first use
RNase-free H2O	5mL
RNase free ultra micro adsorption column	50
Collection tube (2mL)	50

Note: This reagent kit can be stored at room temperature for 12 months without affecting its effectiveness.

Description

This reagent kit uses a centrifugal adsorption column with a specially designed silica matrix membrane. The difference in adsorption capacity between columns is minimal, and the reproducibility is good. Under high salt conditions, RNA efficiently and specifically binds to silica gel adsorption membranes, while maximizing the removal of proteins, inorganic salt ions, and many organic impurities. Under low salt conditions, RNA is eluted. This product adopts a specially designed ultra micro centrifuge column, which can recover trace and ultra micro samples. The general amount of RNA samples that can be processed is 0.1-10μg. This kit is used for purifying and recovering RNA from enzyme reaction solutions (such as DNase treatment, protease treatment, RNA labeling, etc.), and can also be used for purifying RNA extracted from other methods. The purified total RNA has no protein contamination, and the obtained RNA can be used for Northern blot, Dot blot, mRNA extraction, cDNA synthesis, primer extension, differential display, etc.

Application

Suitable for rapid extraction of plant microRNA or separate extraction of microRNA/total RNA.

Features

1. The special gasket free centrifugal column design ensures no liquid residue or contamination after centrifugation. Ensuring high purity of recovered RNA.
2. The special ultra micro centrifuge column design reduces the adsorption membrane area by several times, minimizing the loss of adhesion to the adsorption membrane during recovery. At the same time, it can elute with a minimum volume of 6μL, ensuring high concentration of RNA extraction.
3. The ultra micro centrifuge column is different from other manufacturers in that it adopts our unique hydrophobic and non nucleic acid adsorbing pad base and silicone membrane design. The added base can withstand high-speed centrifugation up to 20000 revolutions per minute without causing the silicone membrane to fall off during high-speed centrifugation. High speed centrifugation ensures the removal of impurities, salt ions, and ethanol, maximizing purity.

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