Manual

Product Number: RNK6901

Shipping and Storage

1. Inappropriate storage at low temperatures (4℃ or -20℃) may cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃-25℃).
2. To avoid the volatilization, oxidation, and pH changes of reagents exposed to air for a long time, the lid of each solution should be promptly closed after use.

Components

Component	50 Preps
Buffer FEA	30mL
PlantAid	3mL
Buffer RW1	40mL
Buffer RW	10mL Add anhydrous ethanol according to the label instructions before the first use
RNase-free H2O	5mL
DNA clearance/RNA adsorption universal column and collection tube	100

Note: This reagent kit can be stored at room temperature for 12 months without affecting its effectiveness.

Description

This product is suitable for rapid extraction of RNA from common plants, common polysaccharide polyphenol plants, and fungi. The exclusive development of genomic DNA clearance/RNA adsorption universal column technology combined with special reagent formula generally does not require DNA enzyme digestion, effectively removing gDNA residue, and obtaining RNA without significant DNA residue. It can be directly used for downstream reverse transcription fluorescence quantitative PCR or high-throughput sequencing library construction experiments.

Application

Suitable for rapid extraction of RNA from common plants, common polysaccharide and polyphenol plants, fungi, and other sources.

Features

1. Completely avoid using toxic reagents such as phenol, chloroform, and beta mercaptoethanol, and do not require steps such as ethanol precipitation.
2. Simple and straightforward, a single sample operation can generally be completed within 12 minutes.
3. Exclusive research and development of genomic DNA clearance/RNA adsorption universal column technology can effectively remove gDNA residues, resulting in extremely high RNA purity, with a typical OD260/OD280 ratio of 2.1~2.2. Generally, DNase digestion is not required and can be used for experiments such as reverse transcription PCR, fluorescence quantitative PCR, high-throughput sequencing library construction, etc.
4. The world's leading adaptability is extremely extensive, successfully extracting hundreds of samples including cotton, roses, Arabidopsis, rice, tobacco, poplar, etc., including some samples that failed to be extracted by domestic and foreign reagent kits. If the effect of particularly complex polysaccharide polyphenols and other samples is not satisfactory, or if other companies fail to produce particularly difficult samples, EASYspin Plus Complex Polysaccharide Polyphenols Plant RNA Extraction Kit can be selected.

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