Manual

Product Number: RNK5201

Shipping and Storage

1. Improper storage of room temperature components at low temperatures (4℃ or-20℃) can cause solution precipitation and affect the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
2. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Component	Storage	RNK5201 50Preps
Buffer RPA	RT	50 ml
Buffer RW1	RT	35 ml
Buffer RW	RT	10 ml Add the specified amount of ethanol as instructed before the first use
RNase-free H2O	RT	5 ml
DNase Buffer	-20℃	1.25 ml×2
RNase free DNase I	-20℃	0.25 ml
RNase free adsorption column RA and collection tube	RT	50

Description

The unique Buffer RPA rapidly cleaves cells and inactivates cellular RNA enzymes. After adjusting the binding conditions with ethanol, RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state. DNase directly digests residual DNA on the column, and then through a series of rapid rinsing centrifugation steps, Buffer RW1 and Buffer RW remove impurities such as cell metabolites and proteins. Finally, low salt RNase free H₂O washes pure RNA off the silica matrix membrane.

Features

1. Completely do not use toxic substances β-Mercaptoethanol/phenol/chloroform does not require steps such as ethanol precipitation.
2. Simplicity, single sample operation can generally be completed within 40 minutes, making it the simplest and fastest reagent kit in the world.
3. The RNA obtained through DNase I column digestion without residual DNase can be directly used for reverse transcription fluorescence quantitative PCR, second-generation sequencing, chip, RACE and other experiments.
4. World leading, it is the most widely adaptable reagent kit among similar products, which can extract plants including rice, corn, wheat, Arabidopsis, tomato, tobacco, and general polysaccharides and polyphenols such as cotton and holly.
5. Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 2.0-2.2 and no DNA residue. It can be directly used for fluorescence quantitative PCR, RT-PCR, chips, second-generation sequencing, Northern blot and other experiments.

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