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2026-08-28Rat malondialchehyche, MDA ELISA Kit
Product Number: ELK20805
Shipping and Storage
- Reagent kit storage: 2-8℃.
- Validity period: 6 months.
Component
| Component | 96T |
| 30× concentrated wash buffer | 20mL×1 |
| Enzyme conjugate | 6mL×1 |
| Microplate (Coated) | 12 wells×8 strips |
| Sample diluent | 6mL×1 |
| Chromogen substrate A | 6mL×1 |
| Chromogen substrate B | 6mL×1 |
| Stop solution | 6mL×1 |
| Standard (16nmol/L) | 0.5mL×1 |
| Standard diluent | 1.5mL×1 |
Description
This kit adopts the double‑antigen sandwich method for measuring rat Malondialdehyde (MDA) levels in specimens. Purified rat MDA antigen is coated onto microplate wells to prepare solid‑phase antigen. MDA sample is added sequentially into the antigen‑coated wells, followed by HRP‑conjugated MDA antigen to form antigen‑antibody‑enzyme‑labeled‑antigen complexes. After thorough washing, chromogenic substrate TMB is added for color development. TMB is converted to blue under catalysis of HRP enzyme, and further turns into final yellow upon acid treatment. Color intensity is positively correlated with MDA concentration in samples. Absorbance (OD value) is read by microplate reader at 450 nm wavelength, and rat MDA concentration in samples is calculated against the standard curve.
Application
This kit is intended for quantitative determination of Malondialdehyde (MDA) concentrations in rat serum, plasma and other related liquid samples.
Testing Scope
0.25nmol/L - 8nmol/L
Specimen requirements
- Extract the specimen as soon as possible after collection, according to relevant literature, and conduct experiments as soon as possible after extraction. If the experiment cannot be conducted immediately, the specimen can be stored at -20℃, but repeated freezing and thawing should be avoided.
- Samples containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP).
