Gene Synthesis at the Core —
Powering Life Science & Biopharma R&D
MEBEP Gene Platform delivers complete molecular biology solutions — gene synthesis, gene cloning, plasmid DNA preparation, mutagenesis & library construction and CRISPR-Cas9 gene editing — for research institutes, biotech companies and pharma worldwide. From sequence design to delivery, every step is standardized and traceable.
Six Service Lines Covering the Full Molecular Biology Workflow
Starting from a single sequence, MEBEP provides an end-to-end service chain — customized gene synthesis, cloning, plasmid preparation, library construction and gene editing — to meet diverse research and industrial R&D needs.
Gene Synthesis
Standard, rapid, express, long-fragment, high-throughput and PCR product synthesis — any gene into any vector.
Gene Cloning
PCR cloning and subcloning with restriction-site-independent seamless cloning for fast access to your target vector.
Plasmid DNA Preparation
Research, transfection, near-GMP and gram-scale preparation with strictly graded endotoxin control.
Mutagenesis & Library Construction
Site-directed mutagenesis and five types of gene libraries for protein engineering, antibody screening and directed evolution.
Other Molecular Services
shRNA expression vectors and bundles, minicircle plasmids, custom mRNA synthesis — one-stop extended services.
CRISPR-Cas9 Gene Editing
ssDNA, sgRNA synthesis and plasmid construction, sgRNA library construction and animal model genotyping.
Gene Synthesis Services
Powered by automated synthesis platforms and standardized production, we synthesize codon-optimized cDNAs, custom-designed DNA sequences or any target gene, and clone them into your vector of choice. Simply provide the DNA or amino acid sequence (please indicate the expression host for optimization) — you receive high-quality gene plasmids with full QC reports.
Standard Gene Synthesis
Any gene into any vector, 100% sequence accuracy
| Gene Length (bp) | Turnaround (Working Days) |
|---|---|
| < 1500 | 5 – 8 |
| 1501 – 3000 | 8 – 12 |
| 3001 – 4000 | 15 – 20 |
| 4001 – 6000 | 20 – 25 |
| > 6000 / Difficult Genes | On Request |
- Sequencing + restriction digest dual verification, re-checked by QC — 100% sequence accuracy
- Seamless cloning into any site of the specified vector, not limited by restriction sites
- In-house gene optimization software for precise expression-system optimization
- Dedicated project manager with regular progress updates
Rapid / Express Gene Synthesis
Fastest 36-hour delivery for fragments ≤ 500 bp
| Gene Length (bp) | Rapid (Working Days) | Express |
|---|---|---|
| < 400 | 4 | 36 hours |
| 400 – 1200 | 5 | 3 days |
| 1201 – 2000 | 7 | 5 days |
Long-Fragment Gene Synthesis
One-step assembly of genes above 6 kb — 100% accurate, mutation-free
With our proprietary cloning technology and long-fragment assembly platform, multiple DNA fragments can be assembled in a single step — greatly saving time and cost versus traditional multi-fragment sequential stitching. All synthesized sequences are 100% accurate with no mutations or errors.
| Gene Length (bp) | Turnaround (Working Days) |
|---|---|
| 6,000 – 10,000 | 30 – 35 |
| 10,000 – 50,000 | On Request |
High-Throughput Gene Synthesis
High-throughput, high-fidelity, automated — 20,000+ genes per month
- Experienced expert design team
- Advanced high-throughput, high-fidelity gene synthesis technology
- Seamless cloning with high cloning success rate
- Well-established project management, ideal for large batches
PCR Product Synthesis
Ready-to-use linear double-stranded DNA, shipped in as few as 2 days
| Length (bp) | Turnaround (Days) | Yield (ng) |
|---|---|---|
| 100 – 500 | 2 – 4 | 500 |
| 501 – 750 | 2 – 4 | 500 |
| 751 – 1000 | 3 – 5 | 500 |
| 1001 – 1250 | 5 – 8 | 500 |
| 1251 – 1500 | 5 – 8 | 500 |
| 1501 – 1750 | 5 – 8 | 500 |
| 1751 – 2000 | 5 – 8 | 500 |
- Every PCR product QC-checked by fragment analyzer
- Short turnaround — shipped in as few as 2 days
- Linear dsDNA, ready to use for simple cloning, construction and engineering
Gene Cloning Services
Seamless cloning is independent of vector restriction sites: target gene fragments can be inserted directly into the designated cloning sites of the specified vector, saving significant time compared with conventional cloning.
PCR Cloning
Amplify the target fragment from template, ligate into the target vector and verify by sequencing
| PCR Cloning Fragment (bp) | Turnaround (Working Days) |
|---|---|
| < 3000 | 6 – 10 |
| 3001 – 4000 | 10 – 13 |
| 4001 – 5000 | 11 – 15 |
| 5001 – 6000 | 13 – 18 |
| > 6000 | On Request |
Subcloning
Re-clone an existing DNA fragment into a new vector for further analysis and re-engineering
| Insert Length (bp) | Turnaround (Working Days) |
|---|---|
| < 3000 | 6 – 10 |
| 3001 – 6000 | 10 – 15 |
| 6001 – 10000 | 15 – 20 |
| > 10000 | On Request |
Plasmid DNA Preparation Services
Application-graded preparation from research, transfection and near-GMP to high-standard gram scale, with strictly graded endotoxin levels (≤ 0.1 / ≤ 0.01 / ≤ 0.005 EU/μg). Monthly extraction capacity: 100,000 mg.
Research-Grade Plasmid DNA
From ¥300 per 100 μg, delivered in as few as 3 days
Suitable for molecular cloning, sequencing, site-directed mutagenesis, Southern blot, library construction, probe synthesis, bacterial transformation and other research applications.
- Automated production, 10 – 500 μg per preparation
- High purity with clean, standard gel bands
- Fastest delivery in 3 days
- Strict quality control
Transfection-Grade Plasmid DNA
Endotoxin ≤ 0.1 EU/μg, predominantly supercoiled
Suitable for transfection of various cell types, viral packaging, antibody or protein production, and animal studies.
- Predominantly supercoiled, endotoxin ≤ 0.1 EU/μg
- Flexible scale from 10 μg to gram level
- Fastest delivery in 4 days
Near-GMP-Grade Plasmid DNA
Produced under GLP standards, endotoxin ≤ 0.01 EU/μg
Suitable for viral vector production for cell therapy such as CAR-T, vaccine development and gene therapy research.
- Produced under strict GLP standard operating procedures, fully traceable
- Supercoiled ≥ 90%, endotoxin ≤ 0.01 EU/μg
- Animal-free, enzyme-free fully automated production with aseptic handling
High-Standard Large-Scale Plasmid Preparation (Gram-Scale)
High-density fermentation from 10 mg to gram level and above — the choice for preclinical vaccine, gene and cell therapy studies
- High-density fermentation with high yield, from 10 mg to gram level and above
- Enzyme-free chromatographic purification, HPLC-verified supercoiled ≥ 90%, endotoxin ≤ 0.005 EU/μg
- Quantitative analysis of bacterial DNA/RNA/protein, customizable QC per request
Molecular Diagnostics Standards — Reference Gene Plasmids
GLP-standard practice, free from exogenous gene contamination
| QC Item | Standard |
|---|---|
| Appearance | Clear, no impurities |
| A260/A280 | Spectrophotometry (1.8 – 2.0) |
| A260/A230 | Spectrophotometry (> 2.0) |
| Restriction digest | Correct band size, no extra bands |
| Genomic DNA / RNA detection | No visible bands by gel electrophoresis |
| Sequencing verification | Sequence verified |
Mutagenesis & Library Construction Services
Site-directed mutagenesis and five types of gene libraries, widely applied in protein engineering, antibody engineering, directed evolution, gene expression optimization, gene knockout, and synthetic / structural biology.
Site-Directed Mutagenesis
Introduce insertions, deletions and point mutations into target DNA — applicable to any sequence, including repeats and high/low-GC regions
| Fragment Length (bp) | Number of Mutations | Turnaround (Working Days) |
|---|---|---|
| < 1000 | 1 – 3 | 5 – 8 |
| 1000 – 3000 | 1 – 3 | 5 – 8 |
| 3000 – 5000 | 1 – 3 | 8 – 10 |
| > 5000 | 1 – 3 | On Request |
Gene Library Construction
Five library types covering everything from protein-function studies to antibody screening
- Site-saturation library: any one or more amino acid positions can be substituted by the other 19 common amino acids (NNK) — ideal for probing protein function, active sites and special conformations
- Scanning library: alanine scanning, etc., to identify residues critical for protein function, interactions and structure
- Random mutation library: controlled error-prone PCR with adjustable mutation frequency of 1 – 20 per kb
- Trimer library: one-to-one codon–amino acid correspondence for precise amino acid ratios, avoiding redundant mutations and stop codons
- Custom library: library design tailored to your research goals
Other Molecular Biology Services
One-stop extended services for gene silencing, long-lasting expression and RNA research.
shRNA Expression Vector Construction
Sustained knockdown of target genes, delivered in 5 – 10 working days
shRNA expression vectors carry antibiotic markers and can continuously suppress target-gene expression in cells for weeks or longer — the preferred solution for long-term gene silencing.
- Stronger in vivo knockdown than synthetic siRNA; compatible with tissue-specific promoters
- Inducible expression systems available to control siRNA expression
- Antibiotic resistance markers allow easy enrichment of silenced cells
- Delivered as plasmid — stable, reproducible, supporting high-throughput applications and gene therapy scenarios
Value Bundle: shRNA Synthesis + Transfection-Grade Plasmid Prep
Discounted synthesis + plasmid extraction — 100 μg transfection-grade plasmid for only ¥500
- Bundled synthesis + transfection-grade plasmid extraction at a better price
- Short turnaround — delivered in as few as 1 week
- Ready to use; advanced high-throughput, high-fidelity synthesis technology
Minicircle Plasmid Construction
Vector backbone removed — persistent, stable expression, purity > 95%
Minicircle plasmids lack the inherent vector backbone, avoiding expression silencing caused by covalent linkage between backbone and expression gene. They offer persistent, stable expression advantages in transfection, DNA vaccine production, viral / antibody production and preclinical animal studies.
| Project Step | Specification | Turnaround (Working Days) |
|---|---|---|
| Parent plasmid by PCR cloning | < 3 kb / 3 kb – 5 kb | 10 – 15 |
| Minicircle delivery | 100 μg / 200 μg | 12 |
| 500 μg / 1 mg | 15 | |
| 2 mg | 20 |
Custom mRNA In Vitro Transcription
Gene synthesis → plasmid preparation → linearization → mRNA synthesis — one-stop service
mRNA of various lengths and functions produced by in vitro transcription, with customization for template and codon optimization, nucleotide modifications and post-transcriptional modifications per downstream application. Widely used in cell transfection, embryo injection, biologics and vaccine development.
CRISPR-Cas9 Gene Editing Services
Full-process companion services for gene editing — from HDR template ssDNA, sgRNA synthesis and plasmid construction, to high-throughput sgRNA library construction and animal model genotyping.
ssDNA Synthesis
High-purity HDR repair templates that significantly improve editing efficiency and accuracy
ssDNA (single-stranded DNA) is commonly used as a homology-directed repair (HDR) template to achieve precise repair and long-fragment knock-in in CRISPR experiments.
| Length (nt) | Turnaround (Working Days) |
|---|---|
| < 400 | 10 – 15 |
| 400 – 2000 | 10 – 15 (sequence dependent) |
| > 2000 | On Request |
- Sequence verified by Sanger sequencing — 100% correct
- PCR-derived, low cytotoxicity, higher homology-repair and knock-in efficiency
- Reorder discount; template plasmid stored frozen for 1 year, shipped within 5 working days
sgRNA Plasmid Construction | sgRNA Synthesis Bundle
Multi-species solutions for higher construction success
Plasmid construction schemes adapted to different species (animal, plant, etc.) to best match your experimental system, delivered in 5 – 10 working days.
CRISPR sgRNA Library Construction
Complete, uniformly distributed coverage — supports high-throughput target screening
Using CRISPR-Cas9 technology, we construct mutant libraries related to a given function. Each custom sgRNA library passes strict QC for complete coverage and uniform distribution, and can be applied to CRISPR knockout, transcriptional activation (CRISPRa) and CRISPRi high-throughput screens.
| Number of sgRNAs | Turnaround (Working Days) |
|---|---|
| < 100 | 10 – 15 |
| > 100 | 15 – 25 |
| > 2000 | 25 – 40 |
Our Quality Assurance System
From production environment to delivery reports — every step is standardized and traceable, and every piece of data is accountable.
Dual Verification
Synthesized genes are verified by both sequencing and restriction digest, then re-checked by the QC department — 100% sequence accuracy.
Automated Platform
High-throughput synthetic biology engineering lab with automated gene synthesis and plasmid extraction platforms — 100,000 mg monthly plasmid extraction, 99.9% delivery rate.
Graded QC
Research, transfection and near-GMP grades with strictly differentiated endotoxin levels (≤ 0.1 / ≤ 0.01 / ≤ 0.005 EU/μg).
GLP Compliance
Near-GMP and molecular diagnostics standards are produced under strict GLP SOPs — free from exogenous gene contamination, fully traceable.
Complete Deliverables
Every order comes with COA, sequencing chromatograms, sequence alignment files and other full technical documentation.
Project Management
A dedicated project manager stays with your project end to end, with scheduled progress updates and prompt responses.
Ordering Process
Four simple steps, with dedicated support throughout.
Submit Your Sequence
Provide the DNA or amino acid sequence (state the expression host), or simply describe your experimental goal
Proposal & Quote
Our technical team assesses sequence complexity and issues turnaround and pricing proposal
Project Execution
Standardized production workflow; your project manager shares regular progress updates
Acceptance & Delivery
Products and QC reports delivered per the agreed specification, with full after-sales support
