Manual

Product Number: C012155

Shipping and Storage

Transport at room temperature and store at 4℃.

Component

Description

Collagenase, derived from Clostridium histolyticum, is a protease that can specifically recognize the Pro-X-Gl-Pro sequence and cleave the peptide bond between the neutral amino acid (X) and glycine (GIy) in the sequence. This sequence appears frequently in collagen. Collagenase is the only protease that can degrade natural collagen fibers with triple helices that are widely present in connective tissue.

This product is I type collagenase, 2125U/mg solld, which can be used for the dissociation of tissues and cells such as epithelium, liver, lung, fat, and adrenal gland.

Definition of Activity

One enzyme activity unit refers to the amount of enzyme required to hydrolyze collagen to produce 1μmol L-leucine within 5 hours at 37°C and pH 7.5.

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