Human ACCPA ELISA Kit
2025-12-11Human VSTM4 ELISA Kit
2025-12-11Human BTLA ELISA Kit
Product Number: ELK020
Shipping and Storage
- Reagent kit storage: 2-8℃.
- Validity period: 6 months.
Component
| Component | 96T |
| 30× concentrated wash buffer | 20mL×1 |
| Enzyme conjugate | 6mL×1 |
| Microplate (Coated) | 12 wells×8 strips |
| Sample diluent | 6mL×1 |
| Chromogen substrate A | 6mL×1 |
| Chromogen substrate B | 6mL×1 |
| Stop solution | 6mL×1 |
| Standard (240ng/mL) | 0.5mL×1 |
| Standard diluent | 1.5mL×1 |
Description
This kit uses the double antibody sandwich method to determine the level of human B/T lymphocyte attenuation factor (BTLA) in the specimen. Coat a microplate with purified human B/T lymphocyte attenuation factor (BTLA) antibody to prepare a solid-phase antibody. Add B/T lymphocyte attenuation factor (BTLA) to the coated micropores in sequence, and then bind with HRP labeled B/T lymphocyte attenuation factor (BTLA) antibody to form an antibody antigen enzyme labeled antibody complex. After thorough washing, add substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The depth of color is positively correlated with the B/T lymphocyte attenuation factor (BTLA) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of human B/T lymphocyte attenuation factor (BTLA) in the sample using a standard curve.
Application
This kit is used to determine the content of B/T lymphocyte attenuation factor (BTLA) in human serum, plasma, and related liquid samples.
Testing Scope
3ng/L -150ng/L
