Human Betatrophin ELISA Kit
2025-12-11
CCK-8 Cell Proliferation and Cytotoxicity Detection Kit
2025-12-11Product Number: ELK026
Shipping and Storage
- Reagent kit storage: 2-8℃.
- Validity period: 6 months.
Component
| Component | 96T |
| 30× concentrated wash buffer | 20mL×1 |
| Enzyme conjugate | 6mL×1 |
| Microplate (Coated) | 12 wells×8 strips |
| Sample diluent | 6mL×1 |
| Chromogen substrate A | 6mL×1 |
| Chromogen substrate B | 6mL×1 |
| Stop solution | 6mL×1 |
| Standard (80μg/L) | 0.5mL×1 |
| Standard diluent | 1.5mL×1 |
Description
This kit uses the double antibody sandwich method to determine the level of human retinol binding protein 4 (RBP-4) in specimens. Coat a microplate with purified human retinol binding protein 4 (RBP-4) antibody to prepare a solid-phase antibody. Add retinol binding protein 4 (RBP-4) to the coated micropores in sequence, and then bind with HRP labeled retinol binding protein 4 (RBP-4) antibody to form an antibody antigen enzyme labeled antibody complex. After thorough washing, add substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The depth of color is positively correlated with retinol binding protein 4 (RBP-4) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of human retinol binding protein 4 (RBP-4) in the sample using a standard curve.
Application
This kit is used to determine the content of retinol binding protein 4 (RBP-4) in human serum, plasma, and related liquid samples.
Testing Scope
1μg/L -40μg/L
