Human BTLA ELISA Kit
2025-12-11Rat Choline ELISA Kit
2025-12-11Human VSTM4 ELISA Kit
Product Number: ELK021
Shipping and Storage
- Reagent kit storage: 2-8℃.
- Validity period: 6 months.
Component
| Component | 96T |
| 30× concentrated wash buffer | 20mL×1 |
| Enzyme conjugate | 6mL×1 |
| Microplate (Coated) | 12 wells×8 strips |
| Sample diluent | 6mL×1 |
| Chromogen substrate A | 6mL×1 |
| Chromogen substrate B | 6mL×1 |
| Stop solution | 6mL×1 |
| Standard (480pg/mL) | 0.5mL×1 |
| Standard diluent | 1.5mL×1 |
Description
This kit employs the double-antibody sandwich method to measure the level of human V-set and transmembrane domain-containing protein 4 (VSTM4) in samples. The microplate is coated with purified human VSTM4 antibodies to form a solid-phase antibody. The sample containing VSTM4 is sequentially added to the wells coated with monoclonal antibodies, followed by binding with HRP-labeled VSTM4 antibodies, forming an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. Under the catalysis of HRP, TMB is converted to blue and further transformed into the final yellow color under acidic conditions. The intensity of the color is directly proportional to the concentration of VSTM4 in the sample. The absorbance (OD value) is measured at a wavelength of 450nm using a microplate reader, and the concentration of human VSTM4 in the sample is calculated based on the standard curve.
Application
This kit is designed for the measurement of protein 4 with V-set and transmembrane domains (VSTM4) in human serum, plasma, and related liquid samples.
Testing Scope
5pg/mL-260pg/mL
