Rat Phosphorus ELISA Kit
2025-12-31
Anaerobic Agar
2026-01-16Rat Calcium (CA) ELISA Kit
Product Number: ELS02
Shipping and Storage
- 2-8℃, stored away from light and moisture
- Validity period: 6 months
Component
| Component | 96-Well Configuration |
| Microplate (96-well, pre-coated) | 12 holes×8 strips |
| Standards | 0.3mL×6 pipes |
| Sample diluent | 6mL |
| HRP-Conjugated Detection Antibody | 10mL |
| 20× washing buffer | 25mL |
| TMB Substrate A | 6mL |
| TMB Substrate B | 6mL |
| Stop solution | 6mL |
| Plate Sealer | 2 sheets |
Note: The concentrations of standard samples (S0-S5) are 0, 2.5, 5, 10, 20, and 40μ mol/mL, respectively.
Description
The reagent kit adopts a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the sample, standard, and HRP labeled detection antibody in sequence to the pre coated micropores with calcium ion (Ca2+) antibodies, incubate and thoroughly wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. The depth of color is positively correlated with the calcium ions (Ca2+) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader and calculate the sample concentration.
Specimen collection and storage
- Serum: Use test tubes without pyrogen and endotoxin, avoid any cell irritation during the operation, collect blood, centrifuge at 3000 rpm for 10 minutes, and quickly and carefully separate serum and red blood cells.
- Plasma: anticoagulant with EDTA, citrate or heparin. Centrifuge at 3000 rpm for 30 minutes and collect the supernatant.
- Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymers.
- Tissue homogenate: Crush the tissue by adding an appropriate amount of physiological saline. Centrifuge at 3000 rpm for 10 minutes and collect the supernatant.
- Storage: If the sample is not tested in a timely manner after collection, please pack it according to a single dose and freeze it at -20℃ to avoid repeated freezing and thawing. Thaw it at room temperature and ensure that the sample is evenly filled and thawed.
Materials required but not supplied
- ELISA reader (450nm)
- High precision sampler and nozzle: 0.5-10μL, 2-20μL, 20-200μL, 200-1000μL.
- 37℃ constant temperature box.
Note
- The reagent kit should be stored at 2-8℃ and equilibrated at room temperature for 20 minutes before use. The concentrated washing solution taken out of the refrigerator will have crystals, which is a normal phenomenon. Heating in a water bath will completely dissolve the crystals before use.
- The Flat noodles not used in the experiment should be immediately put back into the self sealing bag, sealed (low-temperature drying) and stored.
- The S0 standard with a concentration of 0 can be considered as a negative control or blank; When operating according to the instructions, the sample has already been diluted 5 times, and the actual concentration of the sample is obtained by multiplying the final result by 5.
- Strictly follow the time, liquid dosage, and sequence indicated in the instructions for incubation operation.
- Shake all liquid components thoroughly before use.
