Manual

Water Sample DNA Extraction Guide

Product Number: DNK46

Shipping and Storage

All reagents, when store in indicated temperature, are stable for 9 months.

Component

Component	Storage	50T
PES membrane (0.45/0.22um optional)	RT	50
Bead Tube(5mL)	RT	50
Sodium Phosphate Buffer	RT	50mL
MT Buffer	RT	6mL
PPS Solution	RT	13mL
IRS Solution	RT	15mL
PQ Solution	RT	35mL Add indicated ethanol before first use
Buffer WB	RT	13mL Add indicated ethanol before first use
Elution Buffer	RT	10mL
DNA Bind Columns	RT	50

Description

The FastBeat Water DNA Kit quickly and efficiently isolates PCR-ready genomic DNAdirectly from Water samples in less than 40 minutes. Designed for use with Beads-Beating device such as the FastPrep® Instruments from MP Biomedicals，Water organisms population are easily lysed within 40 seconds. Samples are placed into 5.0mL tubes containing 3 kinds of beads, a mixture of ceramic and silica particles designed to efficiently lyse all Water organisms including historically difficult sources such as eubacterial spores and endospores, gram positive bacteria, yeast, algae, nematodes and fungi.

The kit uses a novel and proprietary method to remove high humic acid content including difficult Water types such as compost, sediment, and manure.The isolated DNA has a high level of purity allowing for more successful PCR amplification of organisms from the sample. PCR analysis has been performed to detect a variety of organisms including bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae and Actinomycetes (e.g. Streptomyces).

Important consideration before use

The fill volume in the bead tube after the addition of the Sodium Phosphate and MT Buffers to the sample should allow sufficient air space in the sample tube for efficient FastPrep® Instrument processing. MP Biomedicals recommends using 500 mg of starting material as long as there is between 250 - 500μL of empty space in the tube. Sample loss or tube failure may result from overfilling the bead tube. The bead tube caps must be secure, but not over-tightened, to prevent sample leakage. If the sample is too large for processing in a single tube, divide the sample and process using multiple tubes. The kits have been rigorously tested in the FastPrep® Instrument. A single 40 second run at a speed setting of 6.0 in the FastPrep® Instrument is sufficient to lyse almost all samples. If the user experimentally determines that additional processing time is required, the sample should be incubated on ice in the bead tube for at least 2 minutes between successive FastPrep® Instrument homogenizations to prevent overheating the sample and tube.   

If you use other bead beater device, please follow instruction manual from manufaturer to set appropriate parameter for good performance.

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