RNaseAway Rnase Remover
2024-12-27RNA liquid RNA Blood/Serum/Plasma Kit
2024-12-27Virus Genomic DNA/RNA Fast Kit II（NP）
Product Number: RNK2201
Shipping and Storage
Storage at room temperature for 12 months does not affect the effectiveness of use, and each solution should be covered tightly in a timely manner after use. Poly Carrier can be transported at room temperature and stored at 4 ℃ for one month. It can be stored for a long time at -20 ℃.
Components
| Component | Storage | RNK2201 50 Preps |
| Buffer VLB | RT | 20ml |
| Poly Carrier | -20℃ | 200μl |
| Buffer RE | RT | 25 ml |
| Buffer RW | RT | 10ml |
| RNase-free H2O | RT | 10ml |
| RNase free adsorption column RA and collection tube | RT | 50set |
Description
The virus DNA/RNA extraction kit is suitable for rapidly extracting high-purity virus DNA/RNA from cell-free body fluids, including plasma, serum, ascites, cultured cell supernatant, cerebrospinal fluid, and urine, using a centrifugation adsorption column that specifically binds to virus DNA/RNA and a unique buffer system. This product can meet the simultaneous extraction requirements of most viral RNAs/DNA, such as viral RNAs: HCV (hepatitis C virus), HIV (HIV), and HTLV (human T-lymphotropic virus); Virus DNA: HBV (hepatitis B virus) and CMV (cytomegalovirus), etc. After virus lysis, DNA/RNA selectively adsorbs onto the silica matrix membrane in a highly dissociated salt state (especially equipped with Poly Carrier, which can easily capture trace amounts of nucleic acids from the system). Then, impurities such as salt, cellular metabolites, and proteins are removed through a series of rapid rinsing centrifugation steps. Finally, the pure virus DNA/RNA is eluted from the silica matrix membrane using a low salt elution buffer. The purified viral nucleic acid is free of impurities and PCR inhibitors, and can be directly used for PCR/RT-PCR analysis.
Features
1.No toxic reagents such as phenol are required, and no steps such as ethanol precipitation are required.
2.Time saving, simple, and single sample operation can generally be completed within 20 minutes.
3.Multiple column washes ensure high purity, and the extracted virus DNA/RNA has high purity, stable and reliable quality. It can be used for various routine operations, including PCR/RT-PCR, enzyme digestion, sequencing, Southern hybridization, etc.
