
Product Number: S100045
Shipping and Storage
Store at room temperature, with a shelf life of 12 months.
Component
| Component | S100045 | S100046 |
| Solution A | 20mL | 100mL |
| Solution B | 20mL | 100mL |
| Solution C | 20mL | 100mL |
| Solution D | 20mL | 100mL |
| Solution E | 20mL | 100mL |
| Solution F | 20mL | 100mL |
Description
Masson's trichrome staining, also known as Masson staining, is a classic method for collagen fiber staining. This technique utilizes the differential permeability of anionic biological dyes with varying molecular weights in connective tissues. Smaller molecular weight dyes easily penetrate densely structured, poorly permeable tissues, while larger molecular weight dyes can only enter loosely structured, highly permeable tissues, resulting in distinct coloration of different tissue components.
This product, the Masson Trichrome Staining Solution, is a kit with the following main components: Solution A is a 2.5% potassium dichromate mordant, Solution B and Solution C are mixed in equal volumes to form Weigert's iron hematoxylin stain, Solution D is alizarin red acid fuchsin, Solution E is a 1% phosphotungstic acid solution, Solution F is a 2.5% toluidine blue solution. After staining connective tissue sections with the Masson Trichrome Staining Solution, collagen fibers appear sky blue to bright deep blue, while muscle fibers, cytoplasm, cellulose, keratin white, and other components appear red to purplish red, and red blood cells appear light red.
