Manual

Product Number: F046

Shipping and Storage

Wet ice transportation; Valid for 12 months at 2-8℃; Long term use can also be stored at -20℃, valid for 24 months; Long term storage is recommended to avoid light exposure.

Component

Description

Cell Counting Kit, It is a rapid and highly sensitive detection kit widely used for cell proliferation and cytotoxicity. The detection principle is that in the presence of an electron coupling reagent, the WST-8 compound in the detection reagent is reduced by dehydrogenase in living cells to a water-soluble orange yellow formamide compound, which has a maximum absorption peak at 450 nm. The amount of formaldehyde is directly proportional to the number of live cells, that is, the more and faster the cell proliferation, the darker the color; The greater the cytotoxicity, the lighter the color. For the same cells, there is a linear relationship between the depth of color and the number of cells. WST-8 in this kit has no significant toxicity to cells and can be used for cell proliferation detection induced by exogenous cytokines, as well as cell toxicity detection induced by cytotoxic agents such as drugs, or cell growth inhibition detection induced by some drugs. It is an upgraded version of the MTT detection method.

This reagent kit is easy to use, does not require preparation or dilution, does not require cell collection or washing, and does not require re dissolution of methyl compounds. It is suitable for both adherent cells and suspended cells. And phenol red and serum have no significant effect on the determination of this kit.

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