Manual

Product Number: NST01

Shipping and Storage

-20°C storage, ultra-low temperature ice pack transportation.

Description

Chromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30mM sodium acetate, pH 4.6, 50mM NaCl, 1mM ZnCl2, and 50% glycerol.

Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn²⁺ and/or Ca²⁺. Inhibitors are EDTA, citrate and high concentrations of SDS.

Application

Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA.It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor.

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