Manual

Product Number: EK0631

Shipping and Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Component

Description

This product does not rely on T4 DNA ligase and is not restricted by the restriction sites of the vector and the insert fragment. It uses overlapping fragment recombination. A special enzyme mix can directionally recombine a vector linearized by any method with a PCR fragment (blunt-ended) having 15-25 bp overlapping regions at both ends. It enables highly efficient seamless cloning of 1-5 fragments.

Features

1. Can insert one or multiple long or short PCR-amplified fragments (blunt-ended) into a vector in 15 minutes.
2. Not limited by the availability of restriction sites on the vector and insert, or by the vector's blunt/sticky ends. Cloning can be performed at any site.
3. Seamless cloning; no unwanted base sequences are introduced at the insertion point.
4. Highly efficient and accurate, with a positive clone rate >95%.

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