Endotoxin Removal Beads
2025-11-19
One Step Seamless Cloning Mix (10T)
2025-11-19Product Number: EK0630
Shipping and Storage
Store at -20℃.
Component
| Component | EK0630 |
| 2×Cloning MasterMix | 100µL |
| PUC19 Vector, Linearized(20ng/µL) | 10µL |
| 500 bp Control Insert (20ng/µL) | 10µL |
| ddH2O | 1mL |
Description
Seamless cloning technology is a simple, efficient, and rapid DNA cloning method that enables directional insertion of DNA fragments into any site of a vector. This product does not rely on T4 ligase and is not restricted by restriction enzyme sites of the vector or target fragments. Instead, it directly employs homologous recombination through a specialized enzyme, allowing directional recombination between linearized vectors and PCR fragments with 20-25bp overlapping regions at both ends of the linearized vector. A 5-minute to 15-minute reaction at 50°C can rapidly achieve directional seamless cloning of 1-5 fragments.
Features
- Within 15 minutes, one or more long or short PCR amplification fragments (flat/A end) can be inserted into the vector.
- Cloning can be performed at any site without being limited by the availability of the vector and insertion site, as well as the flat/sticky ends.
- Seamless cloning, insertion points will not introduce unwanted base sequences.
- Efficient and accurate, with a gram-positive rate of over 95%.
