Product Number: BF33
Shipping and Storage
2-8°C
Components
Component | BF33(100mL) |
RIPA Lysis Buffer, Strong | 100mL |
Description
RIPA lysis buffer is a traditional tissue and cell rapid lysis buffer, mainly used to extract soluble proteins from animal tissues and mammalian cells, and can be used to lyse adherent cells and suspended cells. According to the strength of its cracking solution, it can be divided into three categories: strong, medium, and weak. Please refer to Appendix 2 for specific characteristics and differences. Corresponding products can be selected according to experimental needs. RIPA lysis buffer (strong) can effectively extract nuclear, membrane, and cytoplasmic proteins, and the extracted proteins can be used for protein quantification, Western Blot, IP, and other detection and analysis.
The main component of RIPA Lysis Buffer, Strong is 50 mM Tris(pH 7.4),150mM NaCl,1%Triton X-100,1% sodium deoxycholate,0.1% SDS And sodium orthovanadate, sodium fluoride, EDTA, leupeptin, etc.