Chicken Nucleic Acid Detection Kit (Fluorescent PCR Method)
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Product Number: DTK552
Shipping and Storage
Store at -20℃ away from light, with a shelf life of 12 months. After opening, store in the dark at -20℃ without affecting the shelf life; The number of repeated freeze-thaw cycles should not exceed 5 times.
Component
| Component | 50T |
| RT-PCR reaction solution | 375μL |
| Enzyme solution | 250μL |
| Poliovirus type 1, type 2, and type 3 detection solution | 375μL |
| Positive control | 50μL |
| RNAse free water (blank control) | 250μL |
Note: Different batches of reagents cannot be mixed.
Description
This kit uses real-time fluorescence PCR technology and hydrolysis probe technology to achieve qualitative detection of one or more pathogens at the nucleic acid level in the same reaction tube. Easy to operate, no need to open the lid throughout the process to reduce pollution. The instrument software system automatically draws real-time amplification curves to achieve real-time result judgment.
This kit is equipped with an internal standard, which is ribonuclease P (RNaseP) labeled with CY5 fluorescein. The collection, transportation, and extraction of the test sample are monitored through the internal standard to avoid false negatives in the test results.
Application
This kit is used for qualitative detection of poliovirus types 1, 2, and 3 nucleic acids in throat swabs and other samples. The test results are only used for clinical auxiliary diagnosis and cannot be used alone as a basis for confirming or excluding cases.
Poliovirus belongs to the enterovirus genus of the Picornaviridae family, and has three serotypes: type 1, type 2, and type 3. It is mainly transmitted through the digestive tract and can also be transmitted through nasal droplets. Poliovirus often invades the central nervous system, mainly causing poliomyelitis, characterized by damage to the spinal cord anterior horn motor nerve cells and limb flaccid paralysis. As it is more common in children, it is also known as poliomyelitis. The clinical symptoms vary in severity, with most cases being mild or asymptomatic, and a few patients may experience flaccid paralysis. The laboratory testing methods mainly include virus isolation, immunological testing, nucleic acid testing, etc.
Specimen collection
- Sample type: throat swab, etc.
- Collection method: Pharyngeal swab samples: The collection of pharyngeal swab samples shall be carried out in accordance with the pharyngeal swab collection method specified in the Clinical Nursing Practice Guidelines.
- Storage method: After sample collection, it should be tested in a timely manner. The test should be completed within 3 days of storage at 2℃ -8℃, 4 months at -20±5℃, and 12 months at -70℃. The sample should avoid repeated freeze-thaw cycles, with no more than 5 freeze-thaw cycles, and should not be transported in ice packs at low temperatures for more than 3 days.
