The HotStar Taq Mastermix includes the HotStar DNA Polymerase, PCR Buffer, Mg2+, dNTPs, the blue dye, and the stabilizing agent. The HotStar Taq DNA polymerase has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding the extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is activated in 10-minute incubation at 95°C. The activated enzyme maintains the same functionality as Taq DNA polymerase: Has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity. The extending speed is 1 kb/30 sec. There is an "A" on the 3′end. The PCR product can be cloned in a TA vector.
Hot start PCR amplification Specific amplification of complex cDNA and genomic template Amplification from low copy number DNA template Real-Time PCR Multiplex PCR Generation of PCR products for TA cloning
Functional absence of double and single-stranded endonuclease activity; Assayed for amplification from as little as 10 ng of human genomic DNA; Retain full activity at room temperature for one week; No host DNA residue.
Store at -20°C