Manual

Product Number: DTL0157

Shipping and Storage

1. Store below 30°C. It is valid for 12 months.
2. Transport at normal temperature, not suggested over 14 days.
3. Opened but not completely used the all components should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. It is not recommended to repeat the freeze-thaw cycle more than 7 times.
4. Date of manufacture and term of validity: see the label.

Component

Component	48T
PFlu-1/2/3/4 RT-PCR Master Mix	Lyophilized powder ×1 Bottle
Internal Control RT-PCR Master Mix	Lyophilized powder ×1 Bottle
Positive Control	100μL
Negative Control	1mL
Redissolved Diluent	1.5mL

1. Do not mix reagents from different batches.
2. The reaction system is lyophilized powder that contains all components required for fluorescence PCR, including Taq enzyme, reverse transcriptase, primers, probes, dNTPs, and Mg²⁺.

Description

This kit utilizes TaqMan probe-based real-time fluorescent PCR technology and is suitable for detecting type I/II/III/IV nucleic acids extracted from samples such as throat swabs, sputum, and cultures. Each reaction system contains target-specific primers and probes. By collecting the fluorescent signal generated by PCR amplification, qualitative detection of the target nucleic acid can be performed.The kit is provided with an Internal Control（ IC）, which can monitor whether there is PCR inhibitor in the sample to be tested by detecting whether the internal control is normal or not, so as to avoid false negative PCR.

Application

Parainfluenza viruses mainly cause respiratory infections, including upper and lower respiratory tract infections, such as pharyngeal tonsillitis, bronchopneumonia laryngitis, and acute bronchitis. Different subtypes of parainfluenza viruses cause different clinical diseases and clinical manifestations. HPIV-1 and HPIV-2 are the main subtypes that cause acute bronchitis and bronchopneumonia, while pharyngeal tonsillitis and laryngitis are more common in HPIV-3. HPIV-4 can cause community-acquired pneumonia and bronchitis. HPIV1 and HPIV-2 can manifest as nasal congestion, myalgia, and joint pain, and rarely fever, while HPIV-3 often causes fever, cough, rhinitis, etc.

This kit is suitable for the qualitative detection of parainfluenza virus type I/II/III/IV nucleic acids extracted from samples such as throat swabs, sputum, and cultures.

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