2x HotStar Taq Master Mix with dye
2021-09-012x Taq PCR Master Mix with blue dye
2021-09-012x Taq PCR Master Mix with dye
Description:
It contains Taq DNA Polymerase, dNTPs, and an optimized buffer. This Taq Mastermix is provided at 2× concentration and used at 1× concentration by adding the template, primer, and H2O.
Application:
PCR Primer, Extension, Microarray Analysis, High-Throughput PCR
Storage: Store at -20°C
Reaction Mixture Set Up
Template <0.5 μg
Forward Primer (10 μM) 1 μl
Reverse Primer (10 μM) 1 μl
2×TransTaq PCR SuperMix 25 μl
ddH2O to final volume 50 μl
Recommended thermal cycling conditions
94℃ 2-5 min
94℃ 30 sec
50-60℃ 30 sec 30-35 cycles
72℃ 1 min/1-2 kb
72℃ 5-10 min
Notes: no repeated freezing and thawing and split charging for good amplification.
