Avian AP33 (Adhesion Protein 33 kDa) ELISA Kit
2025-12-11Avian CP30 (30 kDa Cysteine Protease) ELISA Kit
2025-12-11Product Number: ELK013
Shipping and Storage
- Reagent kit storage: 2-8℃.
- Validity period: 6 months.
Component
| Component | 96T |
| 30× Concentrated Wash Buffer | 20mL×1 |
| Enzyme Conjugate | 6mL×1 |
| Microplate (Coated) | 12 wells×8 strips |
| Sample Diluent | 6mL×1 |
| Chromogen Substrate A | 6mL×1 |
| Chromogen Substrate B | 6mL×1 |
| Stop solution | 6mL×1 |
| Positive control | 0.5mL×1 |
| Negative control | 0.5mL×1 |
Description
This kit uses the double antibody sandwich method to determine the level of avian AP65 protein (AP65) in specimens. Coat a microplate with purified avian AP65 protein (AP65) antibody to prepare a solid-phase antibody. Add avian AP65 protein (AP65) to the coated micropores in sequence, and then bind with HRP labeled avian AP65 protein (AP65) antibody to form an antibody antigen enzyme labeled antibody complex. After thorough washing, add substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The depth of color is positively correlated with the presence of avian AP65 protein (AP65) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of avian AP65 protein (AP65) in the sample using a standard curve.
Application
This kit is used to determine the content of avian AP65 protein (AP65) in poultry serum, plasma, and related liquid samples.
Testing Scope
3ng/L -180ng/L
