Description

Ribonuclease Inhibitor, Human Placenta is a recombinant human placental protein with specifically inhibits broad-spectrum RNase such as RNase A, RNase B, and RNase C. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H, Taq DNA polymerase, M-MLV Reverse Transcriptase, and T7 RNA Polymerase. The 50kDa protein exerts its inhibitory effect by noncovalently binding to RNases at a 1:1 ratio. The Ki value for binding of Rnase Inhibitor, Human Placenta to RNase is approximately 10-14M.

Source An E.coli strain that carries Ribonuclease Inhibitor gene from human placenta.

Volume activity 40U/ul

Unit Definition of RNase in One unit is defined as the amount of Recombinant Ribonuclease Inhibitor required to inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2’3’-cyclic monophosphate by ribonuclease A.

Storage Temperature -20°C

Storage Buffer 20mM HEPES-KOH(PH7.6), 50mM KCl, 8mM DTT, 50%(v/v) glycerol.

Quality Control

The absence of Endonuclease 1μg of Lambda DNA is incubated with 200 units of Ribonuclease Inhibitor for 16 hours at 37°C. Following incubation, Lambda DNA is visualized as intact on an ethidium bromide-stained agarose gel to verify the absence of visible Endonuclease. RNase Inhibitor, Murine is a 50 kDa recombinant protein of murine origin. The inhibitor specifically inhibits RNases A, B, and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H, or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).

Feature

Improved resistance to oxidation, compared to human/porcine Rnase inhibitor

Ideal for reactions where low DTT concentrations are required (e.g. Real-time PCR)

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