Description

The 2×SYBR qPCR Master Mix include the HotStar DNA Polymerase, PCR Buffer, MgCl2 , dNTPs, SYBR Green I, ROX and the stabilizing agent. The HotStar Taq DNA polymerase which in the mix is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is activated in 10 minute incubation at 95°C. experiments. The Rox in the mix can be used for the correction of the fluorescent signal in the qPCR. The 2×SYBR qPCR Master Mix produces a optimal results in qPCR.

Note

1. Before use, please upside down gently for blending, avoid foam, and short centrifuge.
2. The Master Mix contain the SYBR Green I and the ROX need to avoid the hard light when use and storage.
3. Avoid repeated freezing and thawing, frequently use can be stored at 2-8℃, for long term storage can be store at -20℃.
4. This products can not use for the probe qPCR.

For Protocal pls reference the attachment

Note: The functional activity of the enzyme should to be activated in 10 minute incubation at 95°C

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