Manual

Product Number: ELK060

Shipping and Storage

1. Reagent kit storage: 2-8℃.
2. Validity period: 6 months.

Component

Description

This kit employs the double-antibody sandwich method to detect human parvovirus B19 (PV B19) expression in samples. The microplate is coated with purified human parvovirus B19 (PV B19) antibodies to form solid-phase antibodies, which can bind to PV B19 in the sample. After washing to remove unbound antigens and other components, it combines with HRP-labeled parvovirus B19 (PV B19) antibodies, forming an antibody-antigen-enzyme-labeled antibody complex. Following thorough washing, the substrate TMB is added for color development. TMB is converted into a blue color under the catalysis of HRP enzyme and further transformed into a final yellow color under acidic conditions. The absorbance (OD value) is measured at a wavelength of 450 nm using a microplate reader and compared with the CUTOFF value to determine the presence or absence of human parvovirus B19 (PV B19) in the sample.

Application

This kit is designed for the detection of parvovirus B19 (PV B19) expression in human serum, plasma, and related liquid samples.

Specimen requirements

1. Extract the specimen as soon as possible after collection, according to relevant literature, and conduct experiments as soon as possible after extraction. If the experiment cannot be conducted immediately, the specimen can be stored at -20℃, but repeated freezing and thawing should be avoided.
2. Samples containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP).

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