PG 200 resin 400ml
2024-11-2750bp DNA Ladder marker (5μl/T)
2024-11-29Ni Sepharose High Performance
Product Number: HQ060212001L
Shipping and Storage
4-30℃ in 20% ethanol
Description
Ni Sepharose High Performance is capable of separation and purification by the interactionbetween Ni2+ and certain amino acids (mainly histidine, cysteine, and tryptophan) on theprotein side chain, and is applicable for the separation and purification of His-tag proteinsand biomolecules that interact with Ni2+.
| Matrix | Highly cross-linked 6% sepharose |
| Range of particle size | 25-45µm |
| Mean particle size | 35±5µm |
| Binding capacity | ≥ 40 mg (His-tag protein)/mL (media) |
| pH stability* | 3 - 12 (long-term) 2 - 14 (short-term) |
| Chemical stability** | All common aqueous solutions and buffers; Avoid chelating agents (such as EDTA and EGTA) and reducing agents (such as DTT and DTE) |
| Flow rate | ≥150cm/h |
*: Stability refers to the stability of the media before chelating with metal ions.
| Buffer | 0.05M sodium phosphate, pH 7.4 0.1M Tris-HCl, pH 7.4 0.1M Tris-acetate, pH 7.4 0.1M HEPES, pH 7.4 0.1M MOPS, pH 7.4 0.1M sodium acetate, pH 4 |
| Denaturant | 8M Urea 6M Gua-HCl |
| Detergent | 2% Triton X-100 2% Tween 20 2% NP-40 2% Cholate 1% CHAPS |
| Reducing agent* | 0.005M DTE 0.005M DTT 0.02M β-mercaptoethanol 0.005M TCEP 0.01M reduced glutathione |
| Other additives | 0.5M Imidazole 20% Ethanol 50% Glycerol 0.1M Na2SO4 1.5M NaCl 0.001M EDTA** 0.06M Citrate |
Package
| Product Name | Package | Product Number |
| Ni Sepharose High Performance | 25ml | HQ060212025M |
| Ni Sepharose High Performance | 100ml | HQ060212100M |
| Ni Sepharose High Performance | 500ml | HQ060212500M |
| Ni Sepharose High Performance | 1L | HQ060212001L |
| Ni Sepharose High Performance | 5L | HQ060212005L |
| Ni Sepharose High Performance | 20L | HQ060212020L |
Features
- Fast and simple operation (one-step purification).
- It has wide scope of application, with simple operation, and is applicable for gravity column and pre-packed column (peristaltic pump or chromatography system).
- Multiple options are available: it can be chelated with various metal ions (such as Cu2+, Zn2+, Fe2+, Co2+, Ca2+, etc.) for use.
- Compared with Ni Focurose FF (IDA), it has advantages of low Ni2+ dropout and wide reagent compatibility.
- Small particles, high resolution.
Note: Avoid using phosphate buffers (since there may be precipitates) when chelating with Ca2+.
