Description

SYBR Green I is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allows to use SYBR Green I as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with SYBR Green I - unlabeled primers are enough. Unlike the 10000x SYBR Green I for gel staining purposes, this formulation is specially designed to be used for Real Time PCR.

Features

• Concentration of the dye remains constant and optimal from preparation to preparation - theresults are reproducible over time
• PCR tested preparation - quality guaranteed
• Low fluorescent background - high fluorescence intensity gain

Notes

1. The concentration of SYBR Green I is very important factor for qPCR．The low concentration will bring down the fluorescence signals, then make the low copy template can not be detected.
2. But the hight concentration will inhibit the PCR reaction. So a suitable SYBR concentration is needed, here we recommend optimize the SYBR Green concentration to 0.2x to 1x according the actual case.
3. Increase the concentration of Mg2+ can reduce the inhibition of SYBR Green I in the qPCR reaction. We recommend when use SYBR Green I for qPCR, can increase the Mg2+ concentration make it higher 0.5-3mM than the normal PCR.
4. Always use positive and negative controls when doing qPCR experiments. Thetemperatureprogram for the qPCR amplification does not differ from standard PCR program for the given template and primers. For the detection, FAM or FAM/SYBR channel should be used.

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