Taq Plus DNA Polymerase is a kind of High Fidelity Taq DNA Polymerase mixture of Taq DNA Polymerase and proofreading DNA Polymerase, which allows for the amplification of long templates, up to 30kb, with high fidelity. The two enzymes act synergistically during PCR to generate more accurate and longer PCR products with greater yields compared to Taq DNA Polymerase alone. PCR products, amplified up to 10kb in length with Taq Plus DNA Polymerase, generate a mixture of blunt ends and single base (A) 3' overhang. The products can be used for direct T/A cloning, but their efficiency is not as high as PCR products amplified with Taq polymerase alone.
Storage: Store at –20°C
Taq Plus DNA Polymerase is a special formulation designed for amplifying large fragments. The main component is Taq DNA Polymerase, and Pfu DNA Polymerase are added to enhance the efficiency of the amplification reaction. Theoretically, Taq Plus produces significantly higher yields of PCR products than ordinary Taq Polymerase, especially for fragments >1kb, and can amplify up to 20kb. Taq Plus also contains a proofreading activity that reduces the error rate of Taq Polymerase. Most of the amplified DNA fragments have a 3´A overhanging. However, a small percentage of the amplified DNA fragments are blunt-ended. Taq Plus is suitable as a direct replacement for ordinary Taq Polymerase in most applications.
High fidelity: with an error frequency of 1.6X10-6 during DNA synthesis.
Higher yield: Taq Plus increases the efficiency of the polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 20kb.
One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30min at 74℃.
20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 5mM DTT, 50% glycerol, 0.5% NP40 and 0.5% Tween 20.
10X Reaction Buffer (Mg2+ Plus)
500mM KCl, 100mM Tris-HCl (pH 9.0 at 25℃) and 1% Triton X-100, 100mM (NH4)2SO4, 15mM MgCl2, PCR enhancer
Long PCR (up to 20 kb), PCR cloning, RT-PCR etc.
No contaminating endonuclease or exonuclease activity was detected. Functionally tested in PCR.